EC Number | General Stability | Organism |
---|---|---|
3.4.21.41 | C1rbar undergoes autolytic cleavage | Homo sapiens |
3.4.21.42 | no autolytic cleavage | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.41 | 4-Nitrophenyl-4-guanidinobenzoate | - |
Homo sapiens | |
3.4.21.41 | Ca2+ | reaction with C1s | Homo sapiens | |
3.4.21.41 | diisopropyl fluorophosphate | - |
Homo sapiens | |
3.4.21.41 | leupeptin | competitive | Homo sapiens | |
3.4.21.41 | additional information | high ionic strength inhibits | Homo sapiens | |
3.4.21.41 | phenylmethylsulfonyl fluoride | - |
Homo sapiens | |
3.4.21.42 | C1bar inhibitor | - |
Homo sapiens | |
3.4.21.42 | DFP | - |
Homo sapiens | |
3.4.21.42 | p-nitrophenyl-p'-guanidinobenzoate | - |
Homo sapiens | |
3.4.21.42 | PMSF | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.42 | 0.056 | 0.07 | N-benzyloxycarbonyl-Tyr p-nitrophenyl ester | - |
Homo sapiens | |
3.4.21.42 | 0.069 | - |
N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester | - |
Homo sapiens | |
3.4.21.42 | 1.7 | 2.8 | tosyl-Arg methyl ester | - |
Homo sapiens | |
3.4.21.42 | 2.5 | 5 | benzoyl-Arg ethyl ester | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.41 | additional information | - |
MW of C1r: 166000-188000 Da, gel filtration | Homo sapiens |
3.4.21.42 | 78900 | - |
x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.41 | Homo sapiens | - |
- |
- |
3.4.21.42 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.21.41 | proteolytic modification | autolytic proteolysis involves cleavage of the Arg279-Gly280 bond in the sequence Asp-Ser-Arg-Gly-Trp-Lys | Homo sapiens |
3.4.21.42 | proteolytic modification | activation occurs through cleavage of a single Arg-Ile bond by C1rbar that yields active proteases comprising two chains linked by a single disulfide bride | Homo sapiens |
3.4.21.42 | proteolytic modification | C1s is the proenzyme of C1sbar | Homo sapiens |
3.4.21.42 | side-chain modification | glycoprotein | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.41 | - |
Homo sapiens |
3.4.21.42 | C1s and C1sbar | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.41 | serum | - |
Homo sapiens | - |
3.4.21.42 | serum | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.41 | complement component C1s + H2O | cleavage of a single Arg-Ile bond in the sequence Lys-Gln-Arg-Ile-Ile-Gly | Homo sapiens | complement component C1sbar | - |
? | |
3.4.21.41 | N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | N-acetyl-Gly-Lys + methanol | - |
? | |
3.4.21.41 | N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.41 | N-carbobenzoxy-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | N-carbobenzoxy-Lys + 4-nitrophenyl | - |
? | |
3.4.21.42 | Benzoyl-Arg ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | N-acetyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | N-benzyloxycarbonyl-Tyr-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | Nalpha-acetyl-Tyr ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | Nalpha-tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.42 | Tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.41 | More | C1rbar and the proenzyme C1r are noncovalent dimers, the subunit of C1r has a MW of 53000-85000 Da, SDS-PAGE | Homo sapiens |
3.4.21.42 | ? | x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.41 | additional information | - |
high temperature transition occurs at 53°C, the low-temperature transition varies between 26°C at low ionic strength and 40°C in the presence of 0.5 M NaCl | Homo sapiens |
3.4.21.42 | 49 | - |
10 min, 90% loss of activity | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.41 | 8 | - |
- |
Homo sapiens |
3.4.21.42 | 7 | 8 | - |
Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.42 | 5 | 9.5 | irreversible inactivation below pH 5.0 and above pH 9.5 | Homo sapiens |